技術服務 猴子單克隆抗體制備

服務項目

(Service Items)

周期（周）

Time(weeks)

多肽

Peptide

小分子

Molecular

磷酸基/甲基/乙基化多肽
  Phosphate/Methyl/acetyl-specific

多肽設計與合成

Peptide design & synthesis

多肽設計

Peptide design

~2

可選，免費

多肽合成

Peptide synthesis

√

√

小分子或多肽偶聯

conjugation

√

√

√

免疫階段

Immunization

免疫1只恒河猴

Immunized 1 monkey

~8

√

√

√

ELISA檢測

ElISA detection

√

√

√

cDNA制備

cDNA preparation

逆轉錄制備cDNA文庫

prepare cDNA library by RT-PCR

~1

√

√

√

cDNA 文庫質檢

cDNA library QC check

√

√

√

構建Fab抗體庫

Fab library construction

VH與VL基因獲得

obtained VH and VL   genes

10~15

√

√

√

構建初級抗體庫

primary antibody library construction

√

√

√

構建Fab抗體庫

Fab library construction

√

√

√

噬菌體篩選

phage screening

噬菌體顆粒包裝

phage particles package

10~15

√

√

√

磁珠親和篩選

magnetic beads affinity screening

√

√

√

√

陽性克隆回收基因測序

Recover positive clones and DNA sequencing

√

√

√

抗體表達

Antibody expression

特異性雙鏈基因合成與Fc基因橋接

synthetic of target phage gene and Fc gene

~5

√

√

√

構建真核表達載體

Eukaryotic system expression plasmids   construction

√

√

√

真核表達

Eukaryotic system expression

√

√

√

穩轉細胞系構建

Stably transfected cell line construction

純化與檢測

Purification and detection

標簽純化

Tag affinity purification

~1

√

√

√

特異性親和純化

Antigen specific affinity purification

√

√

√

總周期（月）

Total period (months)

7~9